JBScreen PACT++ is a crystallization screen facilitating systematic pH, anion- and cation testing in the presence of polyethylene glycol (PEG) based on the work of Newman et al. [1].The 96 unique crystallization conditions combine three mini-screens in one:

1. 24-condition PEG/pH screen
2. 24-condition PEG/cation screen
3. 48-condition PEG/anion screen

This systematic approach aims to alter individual components of the crystallization conditions, i.e. pH, anions and cations, independently from the others in order to obtain more information of the precipitation behaviour of the protein.

When JBScreen PACT++ is used along with JBScreen JCSG++, systematic investigation of the precipitation behaviour of the protein can be combined with a sparse matrix screen in order to enhance the success rate of protein crystallization.

Format

Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well

类别：结晶筛
产品信息
产品描述
规格 – 屏幕条件
JBScreen PACT++ 是基于 Newman 等人的工作在聚乙二醇 (PEG) 存在下促进系统 pH、阴离子和阳离子测试的结晶屏幕。 [1]。
96 种独特的结晶条件将三个迷你屏幕合二为一：

1. 24 条件 PEG/pH 筛选
2. 24 条件 PEG/阳离子筛选
3. 48 条件 PEG/阴离子筛网

这种系统方法旨在独立于其他因素改变结晶条件的各个组成部分，即 pH 值、阴离子和阳离子，以获得有关蛋白质沉淀行为的更多信息。

当 JBScreen PACT++ 与 JBScreen JCSG++ 一起使用时，蛋白质沉淀行为的系统研究可以与稀疏矩阵筛选相结合，以提高蛋白质结晶的成功率。

格式

散装 – 24 或 96 种筛查溶液，10 毫升等分试样
HTS – 96 种筛选溶液以深孔模块形式提供，每孔 1.7 ml

References

[1] Newman et al. (2005) Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D 61:1426.

Download technical sheet from below:JBScreen PACT ++ 1 JBScreen PACT ++ 2 JBScreen PACT ++ 3 JBScreen PACT ++ 4 JBScreen PACT++ HTS L

The XP Screen provides 96 crystallization conditions upgraded with the Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) (available here) at 1 mM final concentration. This additive has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms.

TEW was shown to:

• covalently bind and structurally adapt to fit into protein molecules (cgAUS1, PDB code: 4Z12, 4Z13)^[2]
• act as linker in various orientations and thereby create either smaller (abPPO4, PDB code: 4OUA) or larger (lysozyme, PDB code: 4PHI) protein-protein distances^[3,4]
• induce heterogeneous crystallization of two different protein forms in one single crystal (abPPO4, PDB code: 4OUA)^[4]

Our new XP Screen contains TEW in 96 of the most prominent crystallization conditions, providing an environment for TEW to improve crystallization and crystal diffraction quality especially for challenging targets.

SKU： M-CS-350 类别： 结晶筛选和优化, 结晶筛, 新产品
产品信息
产品描述
参考
规格
XP Screen 提供 96 种结晶条件，使用 1 mM 终浓度的 Anderson-Evans 多氧钨酸盐 [TeW6O24]6- (TEW)（可在此处获得）进行升级。这种添加剂已被证明可以促进晶体接触，提高晶体质量，并由于 6 个钨原子进一步提供用于定相的有价值的异常信号。

TEW 被证明：

共价结合并在结构上适应以适应蛋白质分子（cgAUS1，PDB 代码：4Z12、4Z13）[2]
作为各种方向的连接器，从而产生更小（abPPO4，PDB 代码：4OUA）或更大（溶菌酶，PDB 代码：4PHI）的蛋白质-蛋白质距离[3,4]
在一个单晶中诱导两种不同蛋白质形式的异质结晶（abPPO4，PDB 代码：4OUA）[4]
我们新的 XP Screen 在 96 种最突出的结晶条件下包含 TEW，为 TEW 提供了一个环境，以改善结晶和晶体衍射质量，尤其是对于具有挑战性的目标。

[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
[2] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW₆O₂₄O₂(Glu)]^7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286.
[3] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233.
[4] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.

Screen Formulation: Protein Crystal Glue Formulation

MSDS: Protein Crystal Glue MSDS