重组人源组蛋白 H3.2
#M2506S 100 μg
概述
![NEB代理 , 表观遗传学 , 组蛋白](http://www.hamptonresearch.com.cn/wp-content/uploads/2022/11/20221110_636c5ae385b22.png)
来源
其他名称
组蛋白 H3/m、组蛋白 H3/o。
质保声明
浓度
1 mg/ml
组蛋白 H3/m、组蛋白 H3/o。
1 mg/ml
组蛋白 H3.3A、H3F3、H3.3B。
1 mg/ml
Histone H2A combines with Histone H2B to form the H2A/H2B heterodimer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histones are also modified by various enzymes and can act as substrates for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the dimer may be a better substrate for specific enzymes and modifications.
Purified H2A and H2B (NEB #M2502 and NEB #M2505) were denatured, refolded and the dimer was purified by gel filtration
-20°C
20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8.0 @ 25°C
Histone H3.1 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing. The tetramer is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.
Purified H3.1 and H4 (NEB #M2503 and NEB #M2504) were denatured, refolded and the Histone H3/H4 Tetramer was purified by gel filtration
Storage Temperature
-20°C
20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8.0 @ 25°C
Lytechinus variegatus 5SrDNA (1) of 208 bp is used for mononucleosome formation in a gel shift assay.
Plasmid (Litmus 29) containing five copies 5SrDNA is isolated from E. coli NEB 10-beta by a standard purification procedure, digested and 208 bp DNA purified
-20°C
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
停产通知:该产品于2021年12月15日停产。
用杆状病毒表达系统表达人 Dnmt1 cDNA。
未检测到核酸内切酶、外切酶污染。
2,000 units/ml
NEB 提供一系列基因组 DNA(修饰和非修饰两种形式),可用作表观遗传学研究的对照品。所有对照 DNA 均遵循标准基因组纯化程序提取,不含蛋白和 RNA。所提供的对照 DNA 浓度为 100 μg/ml。
1.90
1.85
该产品于 2021 年 11 月 8 日停产
1.87
基因组 DNA 底物
生成胞嘧啶完全甲基化的 DNA
C10H15N3O13P3(无酸)
10 mM 溶液。
481.1(按酸的形式)。
5-甲基-dCTP 不含核酸酶和磷酸酶。