三角烧瓶 锥形瓶
三角烧瓶 锥形瓶三角烧瓶 锥形瓶
三角烧瓶 锥形瓶三角烧瓶 锥形瓶
Alltima 液相色谱柱 88056
80101/N |
3112782/N | All-Guard Cartridge Holder |
HI-081 | N/A | Column Coupler for All-Guard Cartridge Holder (standard column port) |
HI-881 | N/A | Column Coupler for All-Guard Cartridge Holder (Waters type column port only) |
HI-226 | N/A | Analytical Guard Holder Wrench Set- contains a pair of 9/16″ stainless steel wrenches |
C-1000 | 2101342 | Semi-Prep Guard Holder (for 10-22mm id columns) |
81180 | 5129255 | Alltima 3um Amino Column 50×4.6mm |
81183 | 5129258 | Alltima 3um Amino Column 100×4.6mm |
AL-3NH-150DK | Alltima 3um Amino Column 150×2.0mm | |
81190 | 5129265 | Alltima 3um Amino Column 150×4.6mm |
88205 | 5129718 | Alltima 5um Amino Column 150×4.6mm |
96085/N | 5130407/N | Alltima 5um Amino All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88217 | 5129720 | Alltima 5um Amino Column 250×4.6mm |
88217RP | 5133549 | Alltima 5um Amino Column 250×4.6mm RP |
88218 | 5129721 | Alltima 5um Amino Column-W (Waters column port) 250×4.6mm |
88935 | 5129810 | Alltima 3um AQ Column 100×4.6mm |
88940 | 5129811 | Alltima 3um AQ Column 150×4.6mm |
88925 | 5129808 | Alltima 5um AQ Column 150×4.6mm |
88930 | 5129809 | Alltima 5um AQ Column 250×4.6mm |
43853 | 5144675 | Alltima 3um C18 Column 100×1.0mm |
96681/N | 5130648/N | Alltima 3um C18 All-Guard Cartridges (for 2.1mm id columns) 3pk |
43803 | 5126979 | Alltima 3um C18 Column 20×2.1mm |
43849 | 5127000 | Alltima 3um C18 Column 50×2.1mm Do not use, see 5129742/88353 |
88353 | 5129742 | Alltima 3um C18 Column 50×2.1mm |
43821 | 5126988 | Alltima 3um C18 Column 100×2.1mm Do not use, see 5129741/88352 |
88352 | 5129741 | Alltima 3um C18 Column 100×2.1mm |
43852 | 5127002 | Alltima 3um C18 Column 150×2.1mm |
81143 | 5129238 | Alltima 3um C18 Column 150×3.0mm |
43851 | 5127001 | Alltima 3um C18 Column 20×4.6mm |
81412 | 5129318 | Alltima 3um C18 Column 50×4.6mm |
81382 | 5129306 | Alltima 3um C18 Column 100×4.6mm |
11350 | 5124794 | Alltima 3um C18 Column Validation Kit 100×4.6mm (3 columns from 3 different batches) |
81383 | 5129307 | Alltima 3um C18 Column-W (Waters column port) 100×4.6mm |
81387 | 5129308 | Alltima 3um C18 Column 150×4.6mm |
81388 | 5129309 | Alltima 3um C18 Column-W (Waters column port) 150×4.6mm |
AL-3C18-25046 | 5145642 | Alltima 3um C18 Column 250×4.6mm |
50603 | 5127226 | Alltima 3um C18 Rocket 33x7mm |
50605 | 5127227 | Alltima 3um C18 Rocket 53x7mm |
96680/N | 5130647/N | Alltima 5um C18 All-Guard Cartridges (for 2.1mm id columns) 3pk |
88888 | 5136027 | Alltima 5um C18 Column 50×2.1mm |
88370 | 5129746 | Alltima 5um C18 Column 150×2.1mm |
88371 | 5129747 | Alltima 5um C18 Column 250×2.1mm |
96361/N | 5130523/N | Alltima 5um C18 All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
81140 | 5129236 | Alltima 5um C18 Column 150×3.0mm |
81142 | 5129237 | Alltima 5um C18 Column 250×3.0mm |
88383 | 5129752 | Alltima 5um C18 Column 150×3.2mm |
96080SP/N | 5130402/N | Alltima 5um C18 All-Guard Cartridges (for 3.0 – 4.6mm id columns) 10pk |
96080/N | 5130401/N | Alltima 5um C18 All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88889 | 5136051 | Alltima 5um C18 Column 50×4.6mm |
88059 | 5129680 | Alltima 5um C18 Column 100×4.6mm |
AL-5C18-125DF | ALT-5C18-12540 | Alltima 5um C18 Column 125×4.0mm |
88052 | 5129675 | Alltima 5um C18 Column 150×4.6mm |
11541 | 5124810 | Alltima 5um C18 Column Validation Kit 150×4.6mm (3 columns from 3 different batches) |
88051 | 5129674 | Alltima 5um C18 Column-MF (PEEK metal free column hardware) 150×4.6mm |
88053 | 5129676 | Alltima 5um C18 Column-W (Waters column port) 150×4.6mm |
AL-5C18-250DF | N/A | Alltima 5um C18 Column 250×4.0mm |
88056 | 5129678 | Alltima 5um C18 Column 250×4.6mm |
11543 | 5124811 | Alltima 5um C18 Column Validation Kit 250×4.6mm (3 columns from 3 different batches) |
88055 | 5129677 | Alltima 5um C18 Column-MF (PEEK metal free column hardware) 250×4.6mm |
88057 | 5129679 | Alltima 5um C18 Column-W (Waters column port) 250×4.6mm |
88295 | 5129723 | Alltima 5um C18-Nuc Column 250×4.6mm |
AL-5C18-300DF | 5129789 | Alltima 5um C18 Column 300×4.0mm |
AL-5C18-300D | 5129789 | Alltima 5um C18 Column 300×4.6mm |
81102 | 5129213 | Alltima 5um C18 Column 150x10mm |
88063 | 5129681 | Alltima 5um C18 Column 250x10mm |
81106 | 5129215 | Alltima 5um C18 Column 150x22mm |
81105 | 5129214 | Alltima 5um C18 Column 250x22mm |
AL-5C18-10CP2 | Alltima 5um C18 Semi-Prep Guard Cartridges (for 10 – 22mm id columns) 2pk | |
88302 | 5129724 | Alltima 10um C18 Column 150×4.6mm |
88307 | 5129726 | Alltima 10um C18 Column 250×4.6mm |
88308 | 5129727 | Alltima 10um C18 Column-W (Waters column port) 250×4.6mm |
AL-10C18-300DE | N/A | Alltima 10um C18 Column 300×3.9mm |
AL-10C18-300DF | N/A | Alltima 10um C18 Column 300×4.0mm |
970203 | 5130683 | Alltima 10um C18 Prep-Guard Column 33x7mm |
88334 | 5129734 | Alltima 10um C18 Column 250x10mm |
88335 | 5129735 | Alltima 10um C18 Column 250x22mm |
5174010/N | 5174010/N | Alltima C18 All-Guard Cartridge (for 2.1mm id columns) 10pk |
96344/N | 5130511/N | Alltima 5um C18-AQ All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
96432/N | 5130565/N | Alltima 5um C18-LL All-Guard Cartridges (for 2.1mm id columns) 3pk |
88388 | 5129754 | Alltima 5um C18-LL Column 150×2.1mm |
88389 | 5129755 | Alltima 5um C18-LL Column 250×2.1mm |
96285/N | 5130503/N | Alltima 5um C18-LL All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88069 | 5129683 | Alltima 5um C18-LL Column 150×4.6mm |
88099 | 5129695 | Alltima 5um C18-LL Column 250×4.6mm |
81413 | 5129319 | Alltima 3um C8 Column 50×4.6mm |
81392 | 5129310 | Alltima 3um C8 Column 100×4.6mm |
81393 | 5129311 | Alltima 3um C8 Column-W (Waters column port) 100×4.6mm |
81397 | 5129312 | Alltima 3um C8 Column 150×4.6mm |
81398 | 5129313 | Alltima 3um C8 Column-W (Waters column port) 150×4.6mm |
50609 | 5127232 | Alltima 3um C8 Rocket 53x7mm |
88394 | 5144679 | Alltima 5um C8 Column 150×1.0mm |
88394 | 8613481 | Alltima 5um C8 Column 150×1.0mm Do not use, see 5144679/88394 |
96441/N | 5130569/N | Alltima 5um C8 All-Guard Cartridges (for 2.1mm id columns) 3pk |
88372 | 5129748 | Alltima 5um C8 Column 150×2.1mm |
96362/N | 5130524/N | Alltima 5um C8 All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
AL-5C8-100DT | N/A | Alltima 5um C8 Column 100×3.0mm |
81146 | 5129241 | Alltima 5um C8 Column 150×3.0mm |
96081/N | 5130403/N | Alltima 5um C8 All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88072 | 5129684 | Alltima 5um C8 Column 150×4.6mm |
88073 | 5129685 | Alltima 5um C8 Column-W (Waters column port) 150×4.6mm |
88076 | 5129686 | Alltima 5um C8 Column 250×4.6mm |
88077 | 5129687 | Alltima 5um C8 Column-W (Waters column port) 250×4.6mm |
81109 | Alltima 5um C8 Column 150x10mm | |
88081 | 5129688 | Alltima 5um C8 Column 250x10mm |
81110 | 5129219 | Alltima 5um C8 Column 250x22mm |
88312 | 5129728 | Alltima 10um C8 Column 150×4.6mm |
88317 | 5129730 | Alltima 10um C8 Column 250×4.6mm |
88318 | 5129731 | Alltima 10um C8 Column-W (Waters column port) 250×4.6mm |
970227 | 5130704 | Alltima 10um C8 Prep-Guard 33x7mm |
88336 | 5129736 | Alltima 10um C8 Column 250x10mm |
81179 | 5129254 | Alltima 3um Cyano Column 50×4.6mm |
81182 | 5129257 | Alltima 3um Cyano Column 100×4.6mm |
81189 | 5129263 | Alltima 3um Cyano Column 150×4.6mm |
96084/N | 5130406/N | Alltima 5um Cyano All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88065 | 5129682 | Alltima 5um Cyano Column 100×4.6mm |
88180 | 5129712 | Alltima 5um Cyano Column 150×4.6mm |
88182 | 5129714 | Alltima 5um Cyano Column-W (Waters column port) 150×4.6mm |
88189 | 5129715 | Alltima 5um Cyano Column 250×4.6mm |
88191 | 5129716 | Alltima 5um Cyano Column-W (Waters column port) 250×4.6mm |
43813 | 5126983 | Alltima 3um Phenyl Column 150×2.1mm |
81178 | 5129253 | Alltima 3um Phenyl Column 50×4.6mm |
81181 | 5129256 | Alltima 3um Phenyl Column 100×4.6mm |
81188 | 5129262 | Alltima 3um Phenyl Column 150×4.6mm |
43867 | 5127009 | Alltima 3um Phenyl Rocket 53x7mm |
96442/N | 5130570/N | Alltima 5um Phenyl All-Guard Cartridges (for 2.1mm id columns) 3pk |
81147 | 5129242 | Alltima 5um Phenyl Column 150×3.0mm |
96082/N | 5130404/N | Alltima 5um Phenyl All-Guard Cartridges (for 3.0 – 4.6mm id columns) 3pk |
88087 | 5129690 | Alltima 5um Phenyl Column 150×4.6mm |
88088 | 5129691 | Alltima 5um Phenyl Column-W (Waters column port) 150×4.6mm |
88092 | 5129692 | Alltima 5um Phenyl Column 250×4.6mm |
88093 | 5129694 | Alltima 5um Phenyl Column-W (Waters column port) 250×4.6mm |
43884 | 5127020 | Alltima 3um Silica Column 150×2.1mm |
81414 | 5129320 | Alltima 3um Silica Column 50×4.6mm |
81404 | 5129314 | Alltima 3um Silica Column 100×4.6mm |
81405 | 5129315 | Alltima 3um Silica Column-W (Waters column port) 100×4.6mm |
81409 | 5129316 | Alltima 3um Silica Column 150×4.6mm |
81410 | 5129317 | Alltima 3um Silica Column-W (Waters column port) 150×4.6mm |
43877 | 5127016 | Alltima 3um Silica Rocket 53x7mm |
96450/N | 5130573/N | Alltima 5um Silica All-Guard Cartridge (for 2.1mm id columns) 3pk |
96083/N | 5130405/N | Alltima 5um Silica All-Guard Cartridge (for 3.0 – 4.6mm id columns) 3pk |
88123 | 5129697 | Alltima 5um Silica Column 150×4.6mm |
88171 | 5129707 | Alltima 5um Silica Column 250×4.6mm |
88172 | 5129708 | Alltima 5um Silica Column-W (Waters column port) 250×4.6mm |
AL-5-300DF | N/A | Alltima 5um Silica Column 300×4.0mm |
81117 | 5129224 | Alltima 5um Silica Column 150x10mm |
81116 | 5129223 | Alltima 5um Silica Column 250x10mm |
81118 | 5129225 | Alltima 5um Silica Column 250x22mm |
88327 | 5129732 | Alltima 10um Silica Column 250×4.6mm |
970204 | 5130684 | Alltima 10um Silica Prep-Guard 33×7.0mm |
88338 | 5129738 | Alltima 10um Silica Column 250x10mm |
AL-10-300DE | N/A | Alltima 10um Silica Column 300×3.9mm |
88339 | N/A | Alltima 10um Silica Column 250x22mm |
AL-5C18-300DE | N/A | Alltima 5um C18 Column 300×3.9 mm |
Alltima 液相色谱柱 88056
“购买细胞注意事项:
1. 收到细胞后首先观察细胞瓶是否完好,培养液是否有漏液、浑浊等现象,若有上述现象发生请及时和我们联系。
2. 仔细阅读细胞说明书,了解细胞相关信息,如细胞形态、所用培养基、血清比例、所需细胞因子等。
3. 用75%酒精擦拭细胞瓶表面,显微镜下观察细胞状态。因运输问题贴壁细胞会有少量从瓶壁脱落,将细胞置于培养箱内静置培养过夜,隔天再取出观察。此时多数细胞均会贴壁,若细胞仍不能贴壁请用台盼蓝染色测定细胞活力,如果证实细胞活力正常,请将细胞离心后用新鲜培养基再次贴壁培养;如果染色结果显示细胞无活力,请拍下照片及时和我们联系,信息确认后我们为您再免费寄送一次。
4. 建议客户收到细胞后前3天各拍几张细胞照片,记录细胞状态,便于和公司技术部沟通交流。” P4
包装规格: 复苏T25培养瓶(一瓶)或冻存1ml冻存管(两支)
细胞规格: 1*10(5)/ml——1*10(6)/ml
安全水平: 1
细胞种属: 人
组织来源: 盲肠;结直肠腺癌
细胞形态: 上皮细胞样
细胞特性: 悬浮聚集生长,有少数贴壁
细胞融合度: 95%(Viability by Trypan Blue Exclusion)
细胞检测: 细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
“细胞培养三不要:
养科研细胞是生物医学研究的基本功。有三个小经验可以和大家分享一下:
1. 不要烧瓶口。大多数人都喜欢在酒精灯的火焰上关瓶子,觉得这样可以避免污染。不知道大家是不是有培养基怎么越用越发紫的困惑?知道为什么吗?烧瓶口烧的。培养基一般都是用碳酸/碳酸氢跟作缓冲体系。瓶口在火焰上的时候,炽热的空气进入瓶内。塞紧塞子放入冰箱后,空气变冷,压力骤降,培养基中的碳酸就会转化成二氧化碳,释放出来。培养基中的碳酸少了,当然会变碱。如果不烧瓶口的话,你会发现培养液变碱的速度会大大减慢。(当然多多少少还是会有一点越用越碱,因为室温还是比冰箱内的温度高)
其实烧瓶口防止污染纯粹是心理安慰。不妨试一下把手指在火上晃几下,你会发现根本就不会烧疼。如果有细菌的话,这么晃两下也烧不死多少。要想烧死全部细菌,除非把瓶口和塞子烧红。我在国内从来就不烧瓶口。来美国以后发现这里更*,超净台内根本就不点酒精灯。
2. 不要频繁看细胞。对于初学者而言,养细胞就像养孩子一样,有空就要去看看。细胞越是养不好,就越是经常去看。实际上看细胞对细胞的生长没有任何好处,特别是对原代细胞或是免疫磁珠分选后,密度特别低的细胞。培养基中的生长因子是非常有限的。细胞好不容易自分泌一点儿促生长的因子在其周围,形成有利于生长的局部环境。你跑去看细胞,培养瓶这么一晃,把因子都给晃散了。经常可以看到新手一天到晚都在看细胞,细胞老是长不好。老手细胞一扔好几天不管,细胞疯长。
3. 不要怕消化。在传代的时候,初学者往往生怕细胞消化过度,早早地终止消化。细胞没下来就使劲吹灯,吹得满瓶子都是气泡。在我看来,用力吹打对细胞的损伤比消化更大。我师兄在做血管平滑肌原代的时候,37度消化过夜,细胞也没事。我一般是等细胞*变圆后才中止消化。细胞轻轻一晃就能下来。还有,动作要轻柔,尽量不要产生气泡。气泡在破裂时的机械应力相当大,对细胞的伤害也是很大的。” 详见细胞说明书
传代方法: 建议1:2-1:3 两天换液一次
冻存条件: 详见细胞说明书
主要文献: 请具体查阅相关发表文章
细胞传代培养的胰酶消化法:
传代培养:是指细胞从一个培养瓶以1:2或1:2以上的比例转移,接种到另一培养瓶的培养。传代细胞,可根据不同细胞采取不同的方法进行细胞传代。贴壁生长的细胞用消化法传代,部分贴壁的细胞用直接吹打或用硅胶软刮的刮除法传代。悬浮细胞可采用加入等量新鲜培养基后直接吹打分散进行传代,或用自然沉降法加入新培养基后再吹打分散进行传代。
实验步骤:① 入无菌室之前用肥皂洗手,再用75% 的酒精擦拭消毒双手;
② 倒置显微镜下观察细胞形态,确定细胞是否传代及细胞需要稀释的倍数。将培养用液37℃下预热。
③ 超净台台面应整洁,用75%酒精棉球擦拭清洁;
④ 打开超净工作台的紫外灯照射台面20min左右,关闭紫外灯,打开风机清洁空气除去臭氧;
⑤ 点燃酒精灯;取出无菌试管,巴士德吸管和刻度吸管;安上橡皮头;过酒精灯火焰略烧后插在无菌试管内。
⑥ 将培养用液瓶口用75%酒精消毒,过酒精灯火焰后斜置于酒精灯旁的架子上。
⑦ 倒掉培养细胞的旧培养基。酌情可用2-3ml Hanks液洗去残留的就培养基,或用少量胰酶涮洗一下。
⑧ 每个大培养瓶加入1ml胰酶,小培养瓶用量酌减,盖好瓶盖后在倒置显微镜下观察。当细胞收回突起变圆时立即翻转培养瓶,使细胞脱离胰酶,然后将胰酶倒掉。注意勿使细胞提早脱落入消化液中。
⑨ 加入少量的含血清的新鲜培养基,反复吹打消化好的细胞使其脱壁并分散,再根据分传瓶数补加一定量的含血清的新鲜培养基(7-10ml/大瓶;3-5ml/小瓶)制成细胞悬液,然后分装到新培养瓶中。盖好瓶盖,适度拧紧后稍回转,以利于CO2的进入,将培养瓶放回CO2培养箱。
⑩ 对悬浮培养细胞,步骤7-9不做。直接将细胞悬液进行离心去除旧培养基上清,加入新鲜培养基,然后分装到各瓶中。
注意事项
① 传代培养时注意无菌操作并防止细胞间的交叉污染。所有操作尽量靠近酒精灯火焰。每次只进行一种细胞的操作。每种细胞使用一套器材。
② 每天观察细胞形态,掌握好细胞是否健康的标准:健康细胞的形态饱满,折光性好,生长致密时即可传代。
③ 如发现细胞有污染迹象,应立即采取措施,一般应弃置污染的细胞,如果必须挽救,可加含有抗生素的BBS或培养基反复清洗,随后培养基中加入较大量的抗生素,并经常更换培养基。
传代细胞的建系和维持:细胞系的维持通过换液传代再换液、再传代和细胞种子冻存来实现。
对每一个细胞系来说都有其自身的特点,要做好建系的维持必须记录好细胞档案,换液传代和做好细胞系的管理工作。
品牌:Wako
CAS No.:N/A
储存条件:室温
纯度:8 – 12% (as AgNO3) (Calculated on dried basis)
产品名称:10% Silver Nitrate-impregnated Silica Gel 10%硝酸银埋藏硅胶
产品货号:197-11611
产品规格:100g
等 级:for Water Analysis
NKp46蛋白, 种属: Human, 标签: N-His, 表达系统: Expi293NKp46蛋白, 种属: Human, 标签: N-His, 表达系统: Expi293
小角度四口球瓶小角度四口球瓶
玻璃珠灭菌器玻璃珠灭菌器
流式管 5mL 含盖 透明 未灭菌流式管 5mL 含盖 透明 未灭菌
(库存1件)
滤芯吸头 1000μL XL 盒装灭菌 Biosphere滤芯吸头 1000μL XL 盒装灭菌 Biosphere
多肽固相合成管 T型四氟节门 小咀 24/40外磨口多肽固相合成管 T型四氟节门 小咀 24/40外磨口