重组大鼠白介素-4(rrIL-4)

产品介绍

 

CYT-385 重组大鼠白介素-4(rrIL-4)  2μg/10μg/1mg   Prospec

 

 

Background :
  Interleukin-4 (IL4) has a compact, globular fold (similar to other cytokines), stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet.

Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.

 

Description :
  Recombinant Rat IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105 Dalton.

Recombinant Rat IL-4 is purified by proprietary chromatographic techniques.

 

Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
  Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 

Solubility:
  It is recommended to reconstitute the lyophilized IL-4 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
 
  Recombinant Rat IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -180 C. Upon reconstitution, Interleukin-4 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 

Purity:
  Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be
Met-His-Gly-Cys Asn.

 

Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

Biological Activity:
  ProSpec’s Recombinant Rat IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 6.6 x 105 IU/mg.

 

Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of Interleukin-4.

 

Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

 

Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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重组猪白介素-4(rPIL-4)

产品介绍

 

CYT-398 重组猪白介素-4(rPIL-4)  2μg/10μg/1mg   Prospec

 

 

Background :
  Interleukin-4 (IL4) has a compact, globular fold (similar to other cytokines), stabilised by 3 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist. The helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet.

Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.

 

Description :
  Recombinant Porcine IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 110 amino acids and having a molecular mass of 12615 Dalton.
Recombinant Porcine IL-4 is purified by proprietary chromatographic techniques.

 

Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
  Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 

Solubility:
  It is recommended to reconstitute the lyophilized Porcine IL-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
 
  Recombinant Porcine IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution, IL-4 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 

Purity:
  Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp.

 

Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

Biological Activity:
  ProSpec’s Porcine IL-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is < 10 ng/ml, corresponding to a Specific Activity of 1 x 105 IU/mg.

 

Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of Porcine IL-4.

 

Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

 

Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

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重组人白介素-4(rHuIL-4)

产品介绍

 

CYT-211 重组人白介素-4(rHuIL-4)  2μg/10μg/1mg   Prospec

 

 

Background :
  Interleukin (IL)-4, produced primarily by CD4(+) T cells is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.IL-4 has been known as a Th2 cytokine and can act on B cells, T cells, and monocytes.Interleukin (IL)-4 exhibits antitumor activity in rodent experimental gliomas, which is likely mediated by the actions of IL-4 on a variety of immune cells present in and around the tumor masses.

 

Description :
  Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton.

Recombinant IL-4 is purified by proprietary chromatographic techniques.

 

Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
  Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 

Solubility:
  It is recommended to reconstitute the lyophilized IL-4 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
 
  Recombinant IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution, IL-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 

Purity:
  Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp.

 

Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

Biological Activity:
  ProSpec's Recombinant Human Interleukin-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 0.2 ng/ml, corresponding to a Specific Activity of 5×106 IU/mg.

 

Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of rHuIL-4.

 

Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

 

Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 

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重组大鼠白介素-2(rrIL-2)

产品介绍

 

CYT-382 重组大鼠白介素-2(rrIL-2)  5μg/20μg/1mg   Prospec

 

Description :
  Recombinant Mouse IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton.

Recombinant IL-4 is purified by proprietary chromatographic techniques.

 

Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
  Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 

Solubility:
  It is recommended to reconstitute the lyophilized IL-4 in sterile 18Mω-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
 
  Lyophilized IL-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-4 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 

Purity:
  Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ile-His-Gly.

 

Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

Biological Activity:
  ProSpec's Interleukin-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5×105 IU/mg.

 

Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of IL-4.

 

Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.

 

Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

提供美国光谱医学透析袋,wako生化试剂,amresco生化试剂,sigma生化试剂,优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,,洽谈选购!

 

 

Human IL-2 R alpha / CD25 Protein, Fc Tag (HPLC-verified),实验室耗材代理

Human IL-2 R alpha / CD25 Protein, Fc Tag (HPLC-verified)

官方价:
¥19,200.00
产品编号:
ACRILA-H5251-1mg(250ug×4)
品牌:
Acro Biosystems
温馨提醒:
型号规格:
ILA-H5251-1mg/1mg  ILA-H5251-1mg(250ug×4)/1mg:250ug×4  
0
 

    

   

Human IL-2 R alpha / CD25 Protein, Fc Tag (HPLC-verified)Human IL-2 R alpha / CD25 Protein, Fc Tag (HPLC-verified)

重组猪白介素-2(rPIL-2)

产品介绍

 

CYT-397 重组猪白介素-2(rPIL-2)  2μg/10μg/1mg   Prospec

 

 

Description :
  Recombinant Porcine IL-2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15217 Dalton.
Porcine IL-2 is purified by proprietary chromatographic techniques.

 

Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
  The protein (1mg/ml) was lyophilized containing no additives.

 

Solubility:
  It is recommended to reconstitute the lyophilized Porcine IL-2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
 
  Lyophilized Recombinant Porcine IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Porcine IL-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 

Purity:
  Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

 

Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

Biological Activity:
  ProSpec’s Porcine IL-2 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10 MIU/mg.

 

Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of Porcine IL-2.

 

Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.714 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

 

Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 

 

提供美国光谱医学透析袋,wako生化试剂,amresco生化试剂,sigma生化试剂,优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,,洽谈选购!