日度归档：2023年11月27日

重组小鼠粒细胞巨噬细胞集落刺激因子 （rmGM-CSF）

发表于由
产品介绍

 

CYT-222 重组小鼠粒细胞巨噬细胞集落刺激因子 （rmGM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL-6 , IL-7 , EPO and the Prolactin receptors

 
Description :
  Recombinant murine GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.
Murine GM-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  Murine GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4╠0.1.
Solubility:
  It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution GM-CSF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's Murine GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is less then 0.2 ng/ml, corresponding to a Specific Activity of 5×10 6IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of Murine GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！

 

 

重组大鼠粒-巨噬细胞集落刺激因子（rrGM-CSF）

发表于由
产品介绍

 

CYT-395 重组大鼠粒-巨噬细胞集落刺激因子（rrGM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterised by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the connections is a two-stranded anti-parallel beta-sheet. The tertiary structure has a topology similar to that of （pig） growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus .

Granulocyte Macrophage Colony Stimulating Factoris produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL-2 beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

 
Description :
  Recombinant Rat GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton.
Rat GM-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The protein was lyophilized with no additives.

 
Solubility:
  It is recommended to reconstitute the lyophilized GM-CSF in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Rat GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.01 ng/ml, corresponding to a Specific Activity of 1×108 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of Rat GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a calibrated solution of Rat GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！

 

 

 

重组猪粒-巨噬细胞集落刺激因子（rpGM-CSF）

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产品介绍

 

CYT-401 重组猪粒-巨噬细胞集落刺激因子（rpGM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterised by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the connections is a two-stranded anti-parallel beta-sheet. The tertiary structure has a topology similar to that of （pig） growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus .

Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL-6 , IL-7 , EPO and the Prolactin receptors

 
Description :
  Recombinant Porcine GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14381 Dalton.
Porcine GM-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The protein was lyophilized with no additives.

 
Solubility:
  It is recommended to reconstitute the lyophilized Porcine GM-CSF in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Porcine GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Arg-Pro.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec’s Porcine GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation TF-1 cell line is < 20 ng/ml.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.977 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a calibrated solution of Recombinant Porcine GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 

提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！

 

重组人粒-巨噬细胞集落刺激因子-sf9（rHuGM-CSF-Sf9）

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产品介绍

 

CYT-416 重组人粒-巨噬细胞集落刺激因子-sf9（rHuGM-CSF-Sf9）  2μg/10μg/1mg   Prospec

 

 

Description :
  Recombinant Human GM-CSF produced in insect cells is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton.
Recombinant Human GM-CSF is fused to a C-terminal His -tag （6x His） and purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The protein was lyophilized with no additives.

 
Solubility:
  It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
N-terminal methionine has been compley removed enzymatically.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec’s Recombinant GM-CSF is fully biologically active when compared to standard.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells （human erythroleukemic indicator cell line） is < 0.1 ng/ml, corresponding to a Specific Activity of
1 x107 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！

 

 

 

 

重组人粒细胞巨噬细胞集落刺激因子 （GM-CSF）

发表于由
产品介绍

 

CYT-221 重组人粒细胞巨噬细胞集落刺激因子 （GM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  Granulocyte Macrophage Colony Stimulating Factoris produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL-6 , IL-7 , EPO and the Prolactin receptors

 
Description :
  Recombinant Human Granulocyte Macrophage CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton.
Recombinant GM-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4±0.1.
Solubility:
  It is recommended to reconstitute the lyophilized GM-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been compley removed enzymatically.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells （human erythroleukemic indicator cell line） is less then 0.1 ng/ml, corresponding to a Specific Activity of 11.1×10 6IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！

 

 

Quinidine

发表于由

奎尼丁；Quinidine
分子式：C20H24N2O2   分子量：324.42 
简介：奎尼丁  Quinidine是抗心律失常药物，可治疗心律失常，也可用作疟疾的治疗。 
别名：Cinchonan-9-ol, 6′-methoxy-, (9S)- 
物理性状及指标： 
外观：……………………白色无定形或结晶性粉末 
熔点：……………………169℃~174℃ 
溶解性：…………………溶于甲醇，氯仿，乙醇(1 g/36ml)；水(140 mg/l)；苯, DMSO(100mM)；微溶于乙醚和石油醚 
密度：……………………1.22 g/cm3 (预测)
干燥失重：………………≤1.0%
含量：……………………>98.0%
IC50：……………………半数致死剂量 (LD50) 经口 – 大鼠 – 263 mg/kg
储存条件：常温，避光防潮密闭干燥

运输条件：常温运输

生物活性

产品描述

奎尼丁是治疗心律失常和疟疾的抗心律失常药。

靶点

Parasite

体外研究

Quinidine是一种临床抗心律失常药，它影响心肌中的离子电流，并且已被证明是多种细胞类型中K+通道的几类强效阻断剂。浴奎尼丁的应用导致IK的峰值幅度的剂量依赖性降低。在0 mV的阻断IK的KD估计为41μM. Quinidine引起IK衰变的剂量依赖性增加，并且这种效应通过膜去极化得到增强。Quinidine还造成了5 mV的超极化的稳态失活曲线移位和增加复活时。Quinidine不影响灭活测量-发病30 MV 。

体内研究

奎尼丁被迅速吸收，口服后血浆浓度峰值60-90分钟。其他盐(葡萄糖酸盐，聚半乳糖酸盐)吸收较慢，峰值浓度较低。奎尼定与血浆蛋白的结合率约为70- 90%。它经历肝氧化代谢形成n -氧化物，3-羟基，o – dem乙基和2′-喹茚酮。超过一半的患者在治疗的第一年就停止服用奎尼丁因为副作用。这些症状通常包括腹泻、恶心和呕吐，这些症状不一定与血浆浓度高有关。奎尼丁抑制大鼠安非他明的代谢。奎尼丁预处理可使对-羟基安非他明在24小时、48小时、7.2小时和24.1%车辆控制水平下的排泄量显著降低，同时使安非他明在24小时至48小时、542%的控制水平下的排泄量显著增加。

相关产品推荐

MB1442

氢化奎宁定;双氢奎尼丁

用途及描述：科研试剂，广泛应用于分子生物学，药理学等科研方面。严禁用于人体。直接抑制心肌Na+内流，显著延长心肌不应期抑制心肌自律性和传导性，从而有助于消除折返。降低心肌应激性，提高致颤阈。此外，还可抑制心肌细胞Ca2+内流和阻滞血管?受体，出现负性肌力作用和血管扩张，双重影响使血压明显降低。用药早期，其血药浓度低时，主要表现对植物神经的间接作用，即抗胆碱(阿托品样)作用；当血药浓度达稳态时，则直接作用占优势。用于房颤和房扑电转复的准备和转窦性心律后预防复发；减少或预防阵发性室上性心动过速和室性心动过速的复发。

储液配置：

Quinidine

1 mg

5 mg

10 mg

1 mM

3.0824 mL

15.4121 mL

30.8242 mL

5 mM

0.6165 mL

3.0824 mL

6.1648 mL

10 mM

0.3082 mL

1.5412 mL

3.0824 mL

经典实验操作（仅供参考）

动物实验
  • 奎尼丁溶解在50%乙醇中
  • 大鼠:3只大鼠被放在单独的代谢笼子里，可以自由获得食物和水。24小时后收集尿液(0小时)，大鼠接受以下治疗:(1)不治疗;(2) 80 mg奎尼丁/kg (po) 1.0 mL/kg, 50%乙醇(1.0 mL/kg)。两小时后，所有3只大鼠均以1.0 mL/kg的剂量接受15毫克安非他命(po)。然后在服用奎尼丁后的24小时和48小时收集尿液。这个过程重复三次。收集尿液后，测量尿液体积和pH值，并储存至分析。

【注意】 
●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。 
参考文献： 
1. Efficacy and safety of quinidine therapy for maintenance of sinus rhythm after cardioversion. A meta-analysis of randomized control trials.
2. Quinidine syncope: paroxysmal ventricular fibrillation occurring during treatment of chronic atrial arrhythmias

3. Incidence and clinical features of the quinidine-associated long QT syndrome: implications for patient care.

我司所售出产品仅供于科研研究用途（非临床科研研究），每次销售产品行为都适用于我司网上所列明的。

商品货号：MB1702
CAS 号：56-54-2
英文名字：奎尼丁
质量标准：>98.0%,BR
分子式：C20H24N2O2