日度归档：2023年11月27日

重组人干扰素-α 1b （rHuIFN-alpha 1b）

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产品介绍

 

CYT-283 重组人干扰素-α 1b （rHuIFN-alpha 1b）  20μg/100μg/1mg   Prospec

 

 

Background:
  At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

 
Description :
  Recombinant Human Interferon alpha-1b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton.
The Interferon-alpha 1b gene was obtained from human leukocytes.
The IFN-alpha-1b is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  Lyophilized from a （1mg/ml） solution in containing 20mg HSA and 7.5mg sodium chloride.
Solubility:
  It is recommended to reconstitute the lyophilized IFN alpha-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized IFN-alpha-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's Interferon-alpha 1b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 108 IU/ mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of IFN-alpha 1b.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 
Latest Publications:
  1. [Gene modification and high prokaryotic expression of porcine interferon alpha-1]

Sheng Wu Gong Cheng Xue Bao 2004 Mar;20（2）:291-4

2. [Translational control of human interferon alpha 1 gene expression in E.coli]

Zhongh ua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Dec;16（4）:315-8

3. Differential effects of angiostatin, endostatin and interferon-alpha（1） gene transfer on in vivo growth of human breast cancer cells.

Gene Ther 2002 Jul;9（13）:867-78

4. High-yield expression and purification of human interferon alpha-1 in Pichia pastoris.

Protein Expr Purif 2001 Aug;22（3）:381-7

5. Unusual effect of clusters of rare arginine （AGG） codons on the expression of human interferon alpha 1 gene in Escherichia coli.

Int J Biochem Cell Biol 1997 Apr;29（4）:659-66

6. Extracellular matrix remodelling in a murine mammary adenocarcinoma transfected with the interferon-alpha 1 gene.

J Pathol 1997 Jan;181（1）:116-23
 

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重组人干扰素-β 1a （rHuIFN-β 1a）

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产品介绍

 

CYT-236 重组人干扰素-β 1a （rHuIFN-β 1a）  2μg/10μg/1mg   Prospec

 

 

Background:
  One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells.
IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma.

 
Description :
  Recombinant Human Interferon-Beta 1a produced in CHO（Chinese Hamster Ovarian） cells is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500 Dalton.
IFN-beta-1a is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  Lyophilized from 1mg/ml solution containing 91mg Human Albumin, and 620mg mannitol, and 9mg Sodium Acetate.
Solubility:
  It is recommended to reconstitute the lyophilized Interferon beta-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized IFN-beta-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-beta-1a should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Tyr-Asn-Leu.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's Interferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay （human “Wish” cell line and VSV virus or the monkey VERO cell line with EMCV virus） was found to be 270 x 106 IU/ mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of IFN-beta-1a.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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重组人干扰素-β 1b （rHuIFN-β1b）

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产品介绍

 

CYT-234 重组人干扰素-β 1b （rHuIFN-β1b）  2μg/10μg/1mg   Prospec

 

 

Background:
  One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 .

 
Description :
  Recombinant Human IFN-beta 1b produced in E.Coli is a single, non-glycosylated mutein （variant form） of human Interferon beta-1b polypeptide chain containing 165 amino acids and having a molecular mass of 18510.86 Dalton. The Interferon beta gene was cloned from human fibroblasts and altered to substitute Serine for the Cysteine residue found at position 17.
IFN-beta-1b is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  1mg/ml solution containing 50mg Human Albumin & 50mg dextrose.
Solubility:
  It is recommended to reconstitute the lyophilized Interferon beta-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Interferon beta-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution,liquid form of IFN-beta-1b should be stored at 4 C up to 3 weeks and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's Interferon beta-1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay （human “Wish” cell line and VSV virus or the monkey VERO cell line with EMCV virus） was found to be 32 x 10 IU/mg. 6 IU/ mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of IFN-beta-1b.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.493 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

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重组人干扰素- alpha 2a（rHuIFN-a 2a）

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产品介绍

 

CYT-204 重组人干扰素- alpha 2a（rHuIFN-a 2a）  20μg/100μg/1mg   Prospec

 

 

Background:
  At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
 
Description :
  Recombinant Human IFN-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton.
Interferon-alpha 2a gene was obtained from human leukocytes.
The IFN-a is purified by proprietary chromatographic techniques.
 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.
 
Formulation:
  Recombinant IFN-a is lyophilized from （1mg/ml） solution containing 7.21 sodium chloride and 0.77mg ammonium acetate.
Solubility:
  It is recommended to reconstitute the lyophilized IFN-alpha-2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
  Lyophilized IFN-alpha-2a although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-2a should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.
 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha . N-terminal methionine has been compley removed enzymatically.
 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
  ProSpec's IFN-alpha 2a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 108.
 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of rHuIFN-α 2a.
 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of IFN-alpha（2a） as a Reference Standard.
 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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纸质冻存盒 彩色 翻盖型 瑞博品牌,实验室耗材代理

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纸质冻存盒 彩色 翻盖型 瑞博品牌

官方价：
¥10.00
产品编号：
OTVRBDCH-9×9-BM
品牌：
其它
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型号规格：
RBDCH-10×10-C-Case／10×10 100格 100个/箱  RBDCH-4×4-C／4×4 16格 50mL 白色天地盖  RBDCH-7×7-C／7×7 49格 单个  RBDCH-9×9-BM／9×9 81格 单个 防水  RBDCH-9×9-C-Case／9×9 81格 80个/箱  
2896
 

    

   

纸质冻存盒 彩色 翻盖型 瑞博品牌纸质冻存盒 彩色 翻盖型 瑞博品牌

重组人肝细胞生长因子（rHuHGF）

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产品介绍

 

CYT-244 重组人肝细胞生长因子（rHuHGF）  2μg/10μg/1mg   Prospec

 

 

Background:
  Hepatocyte Growth Factor （HGF） is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis

 
Description :
  Recombinant Human HGF produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an -chain of 463 amino acids and -chain of 234 having a total molecular mass of 80017 Dalton.
HGF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The sterile protein powder is lyophilized with no additives.
Solubility:
  It is recommended to reconstitute the lyophilized HGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution HGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  Agrees with the sequence of native human HGF.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec's Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of HGF.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！