实验用电加热圈 结晶皿加热管实验用电加热圈 结晶皿加热管
| PKA-311 | 重组人周期蛋白-C（rCCNC） | 2μg/10μg/1mg | Prospec |
| Background : | |
| rCCNC （cyclin C） is a member of the cyclin family of proteins. This protein interacts with cyclin-dependent kinase 8 （CDK8） and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this protein peaks in the G1 phase of the cell cycle. It has been shown that a non-cdk8-associated cellular pool of CCNC combines with cdk3 to stimulate pRb phosphorylation at S807/811 during the G0/G1 transition, and that this phosphorylation is required for cells to exit GO efficiently. Other results have shown that a marked elevated immunoreactivity of CCNC was found both in neurons and astrocytes in Alzheimer's disease （AD）. Increased levels of CCNC RNA were detected by ribonuclease protection assay （RPA） in severe AD cases and colocalization of CCNC and its preferred binding partner, CDK8, was only observed in astrocytes but not in neurons.
|
|
| Description : | |
| Recombinant CNCC is expressed in E. coli as a full-length protein fused to a proprietary 16-Kd tag （a 6xHis tag is located at the very N-terminal end）.
|
|
| Formulation: | |
| Recombinant CDK8 is supplied at 0.1mg/ml in a buffer containing 25 mM HEPES, pH 7.9, 125mM KCI, 0.5% TX-100, and 50% glycerol.
|
|
| Storage: |
|
| Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.
|
|
| Application: | |
| Recombinant human CNCC can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.
|
|
| Purity: | |
| Greater than 90% （SDS-PAGE）.
|
|
| Usage: | |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！
实验用电加热圈 结晶皿加热管实验用电加热圈 结晶皿加热管
| PRO-300 | 重组细胞周期素依赖性激酶抑制剂1（P21-WAF1） | 2μg/5μg/10ug | Prospec |
| Background: | |
| p21/WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21/WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA （proliferating cell nuclear antigen） and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.
|
|
| Description : | |
| Recombinant P21/WAF1 shows a band at 47kDa on SDS-PAGE commassie blue stained gel. Recombinant P21/WAF1 is purified by proprietary chromatographic techniques.
|
|
| Physical Appearance: | |
| Sterile filtered liquid.
|
|
| Formulation: | |
| 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.
|
|
| Stability: |
|
| Store vial at -20Co to -80Co. When stored at the recommended temperature, recombinant P21/WAF1 is stable for 12 months. Please avoid freeze-thaw cycles.
|
|
| Purity: | |
| Greater than 90.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.
|
|
| Dimers and aggregates: | |
| Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.
|
|
| Endotoxin: | |
| Less than 0.1 ng/µg （IEU/µg） of Human P21/WAF1.
|
|
| Usage: | |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！
实验用电加热圈 结晶皿加热管实验用电加热圈 结晶皿加热管
、
| PRO-303 | 重组人增殖细胞核抗原（rHuPCNA） | 5μg/20ug | Prospec |
Synonyms:
Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
Introduction:
Proliferating cell nuclear antigen （PCNA）, a protein of 30 kDa molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis.
Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity （< 10 % of SLE patients）. However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.
Description:
PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29,794 Dalton.
PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Source:
Sf9 insect cells.
Physical Appearance:
Sterile Filtered colorless solution.
Formulation:
Recombinant Human PCNA is supplied at a of 1mg/ml in16mM HEPES buffer pH-8, 400mM NaCl, and 20% glycerol.
Immunological functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test （checker-board analysis of positive/negative sera panels, immuno-dot analysis with validated SLE patient sera
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration:
0.5-1.1 µg/ml （depending on the type of ELISA plate and coating buffer）.
Suitable for biotinylation and iodination.
Storage Conditions:
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！
、
实验用电加热圈 结晶皿加热管实验用电加热圈 结晶皿加热管
、
| CYT-431 | 重组人巨噬细胞移动抑制因子（rHuMIF） | 5μg/25μg/1mg | Prospec |
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录
、
红液棒形温度计红液棒形温度计
、
| CYT-281 | 重组人成纤维细胞生长因子21（rHuFGF 21 , His） | 2μg/10μg/1mg | Prospec |
Synonyms:
Fibroblast growth factor 21, FGF-21.
Introduction:
The FGFs are a family of more than 20 small （~17–26 kDa） secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors （FGFRs） 1, 2, or 3. A fourth closely related tyrosine kinase receptor （FGFR4） was able to bind the FGFs but did not lead to a mitogenic response.
FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors （FGFRs）: FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in β cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal β cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-19, has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.
FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.
Description:
Fibroblast Growth Factor -21 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids and having a molecular mass of 21 kDa. The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the human FGF21 without signal sequence and contains 14 a.a. His tag at N-terminal.
The FGF-21 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered white lyophilized powder.
Formulation:
Filtered （0.4 µm） and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5.
Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-21 Human Recombinant sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized FGF-21 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity:
Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence:
MRGSHHHHHH GMASHPIPDS SPLLQFGGQV RQRYLYTDDA TEAHLEIR EDGTVGGAAD QSPESLLQLK ALKPGVIQIL GVKTSRFLCQ RPDGALYGSL HFDPEACSFR ELLLEDGYNV YQSEAHGLPL HLPGNKSPHR DPAPRGPARF LPLPGLPPAP PEPPGILAPQ PPDVGSSDPL SMVGPSQGRS PSYAS.
Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！
、