JBScreen LCP-MiTeGen不同类型 LCP 脂质中膜蛋白结晶筛选
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JBScreen LCP
JBScreen LCP is a crystallization screen designed for efficient screening of crystallization conditions in the Lipidic Cubic Phase (LCP), which has become the method of choice for membrane protein crystallization in different types of LCP lipids.
JBScreen LCP
JBScreen LCP 是一种结晶筛,专为有效筛选脂质立方相 (LCP) 中的结晶条件而设计,已成为不同类型 LCP 脂质中膜蛋白结晶的首选方法。
Product Information
- Product Description
- Technical Specifications
JBScreen LCP is a crystallization screen designed for efficient screening of crystallization conditions in the Lipidic Cubic Phase (LCP), which has become the method of choice for membrane protein crystallization in different types of LCP lipids. The 96 conditions of JBScreen LCP result from data mining of 192 integral membrane proteins, that were successfully crystallized by the in meso method and have yielded structures [1]. The screen is ordered by type and concentration of the precipitant and is free of cacodylate.Representation of the events proposed to take place during the crystallization of an integral membrane protein from the lipid cubic mesophase. Image from [1], used by courtesy of Prof. Martin Caffrey, Trinity College Dublin, Ireland.
Formats
Bulk – 4 x 24 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well
Reference [1] Caffrey (2015) A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes. Acta Cryst F 71:3.
Download technical sheets from these Jena links:JBScreen LCP Screen Formulation JBScreen LCP HTS Screen Formulation Description how the JBScreen buffers are prepared
类别:结晶筛选和优化, 结晶筛选, 膜蛋白晶体筛选, 膜蛋白结晶
产品信息
产品描述
技术规格
JBScreen LCP 是一种结晶筛,专为有效筛选脂质立方相 (LCP) 中的结晶条件而设计,已成为不同类型 LCP 脂质中膜蛋白结晶的首选方法。 JBScreen LCP 的 96 种条件来自对 192 种完整膜蛋白的数据挖掘,这些蛋白通过内消旋方法成功结晶并产生结构 [1]。筛网按沉淀剂的类型和浓度排序,不含二甲胂酸盐。
表示在从脂质立方中间相结晶完整膜蛋白期间提议发生的事件。图片来自 [1],由爱尔兰都柏林三一学院的 Martin Caffrey 教授提供。
格式
散装 – 4 x 24 筛选溶液,每 10 毫升等分试样
HTS – 96 种筛选溶液以深孔模块形式提供,每孔 1.7 ml
参考文献 [1] Caffrey (2015) 对脂质立方相或细观方法结晶膜和可溶性蛋白质和复合物的综合评论。 Acta Cryst F 71:3。
从这些 Jena 链接下载技术表:
JBScreen LCP 屏幕配方 JBScreen LCP HTS 屏幕配方 描述 JBScreen 缓冲区是如何准备的