 几分钟之内将抗体及融合蛋白完全去糖基化

75℃ 10 分钟。

 无糖苷外切酶污染，无糖苷内切酶F1、F2 和 F3 活性，无蛋白水解活性。

147,000 daltons。

40,000,000 units/ml。

 65℃ 10 分钟。

如果有唾液酸存在，先去除唾液酸。

10X GlycoBuffer 1 反应缓冲液

136,000 daltons。

200,000 units/ml。

55℃ 10 分钟。

X=（H 或寡糖）

140,000 daltons。

50,000 units/ml。

65℃ 10 分钟。

 从糖蛋白和糖肽链上切除复杂二、三支链 N-连接糖原

糖苷内切酶 F3 克隆自和平空间站伊丽莎菌（Elizabethkingia miricola，formerly Flavobacterium

 10X GlycoBuffer 4 反应缓冲液

 38,800 daltons

 无糖苷外切酶污染，无蛋白水解活性。

 1 单位指 10 μl 的反应体系中，37℃ 条件下，1 小时从 10 μg 猪纤维蛋白原中除去超过95% 的碳水化合物所需要的酶量。

 8,000 units/ml

 65℃ 10 分钟。

 从糖蛋白和糖肽链上切除复杂二支链 N-连接糖原

 10X GlycoBuffer 4 反应缓冲液

 39,800 daltons。

 无糖苷外切酶污染，无蛋白水解活性。

1 单位指 10 μl 的反应体系中，37℃ 条件下，1 小时从 10 μg 猪纤维蛋白原中除去超过95% 的碳水化合物所需要的酶量。

8,000 units/ml。

 65℃ 10 分钟。

有关该产品特性和应用的相关文献请登陆 www.neb-china.com，www.neb.com。

  Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in

     various glycosphingolipids.

  Oligosaccharide and ceramide remain intact, leaving them suitable for further analysis. 

  This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to

     the scientific community in the hopes of enabling the discovery of new and innovative applications.

    These enzymes have interesting properties and unique specificities.

Endoglycoceramidase I (EGCase I) catalyzes the hydrolysis of the β-glycosidic linkage between

oligosaccharides and ceramides in various glycosphingolipids. The enzyme leaves the oligosaccharide

and ceramide intact, keeping them suitable for further analysis. The enzyme efficiently releases

oligosaccharides from ganglio- and globo-type glycosphingolipids, certain type of cerebrosides

(glucosylcerebrosides), and exhibits low activity towards galactocerebrosides (See Reference 1,

FAQ #3). EGCase I does not hydrolyze sulfatides nor psychosine. 

EGCase I is isolated from a strain of E. coli, which contains the cloned EGCase I gene from Rhodococcus triatomea.

 1X EGCase I Reaction Buffer. Incubate at 37°C

10X EGCase I Reaction Buffer

1X EGCase I Reaction Buffer
0.1% Triton® X-100
50 mM sodium acetate
(pH 5.2 @ 25°C)

Apparent: 50 kDa

One unit of R. triatomea EGCase I was defined as the amount of enzyme required to hydrolyze 1 μmol of

ganglioside GM1a per minute at 37°C.

Unit Assay Conditions
Two fold dilutions of EGCase I are incubated with 10 nmoles of GM1a substrate in 1X EGCase I Reaction

Buffer in a 10 µl reaction. The reaction mix is incubated for 5 minutes at 37°C. Released glycans are

labeled with 2-AB, and reaction products are analyzed by ultra-performance hydrophilic interaction liquid

chromatography with fluorescence detection.

65°C for 20 min.

 1. Albrecht, S. et al. (2016). Analytical Chemistry. 88, 4795-4802.

胎牛血清。

64 kDa。

10 mg/ml。

RNase B是一种高甘露糖蛋白，可作为糖苷内切酶切割N-连接糖链的阳性对照。RNase B有一个N-连接糖基化位点，可以用于SDS-PAGE凝胶电泳分析。

牛胰

Milli-Q™水

17,000 Da（去糖基后13,683 Da）

250 μg足够用于约20次反应。