EMnetik 24微粒处理器，配套PCR纯化试剂盒和质粒纯化试剂盒使用。采用电磁场技术，以“无机械运动”的方式重新定义了磁珠混匀、吸附的全过程，从而极大的缩减了核酸纯化所需的时间和人力。

Beckman贝克曼 纯化磁珠 核酸纯化试剂5ml（A63880）

1、双链和单链DNA都能得到纯化

2、有效去除dNTP、引物、引物二聚体、盐离子和其它杂质

2、200bp以上的核酸回收率高达90%以上，核酸长度最小要求100bp

3、纯化后的产物在4℃温度下保持7天不被降解

4、适用于PCR体系纯化、酶切和连接反应体系纯化

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features

• High recovery of amplicons, greater than 100 bp
• Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
• Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
• Efficient recovery of double stranded and single stranded DNA templates
• Consistent recovery throughout the kit’s 12 month shelf life
• Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Process Overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NX^P and FX^P for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

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