重组人巨噬细胞集落刺激因子 （rHuM-CSF）

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CYT-308 重组人巨噬细胞集落刺激因子 （rHuM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  Macrophage colony stimulating factor （M-CSF） is one of the glycoproteins called colony-stimulating factors （CSFs）.A mononuclear phagocyte colony-stimulating factor （M-CSF） synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo

 
Description :
  Recombinant Human M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD
Recombinant M-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The lyophilized protein （1mg/ml） was lyophilized with 0.1gr HSA and 0.6gr Manntiol.

 
Solubility:
  It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution M-CSF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec’s M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 105 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of M-CSF.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of M-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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