重组大鼠白介素-3（rrIL-3）

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CYT-383 重组大鼠白介素-3（rrIL-3）  2μg/10μg/1mg   Prospec

 

 

Background :
  Interleukin-3 （IL-3） is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases

 
Description :
  Recombinant Rat IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 15783 Dalton.
Recombinant Rat IL-3 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  Lyophilized from a 1mg/ml solution in water containing 5mM sodium citrate pH-4.

 
Solubility:
  It is recommended to reconstitute the lyophilized IL-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Rat IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec’s rrIL-3 is fully biologically active when compared to standard.
The ED50 as determined by the dose-dependant stimulation of thymidine uptake by murine MC-9 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 1 x 105 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of Recombinant Rat IL-3.

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of Rat IL-3 as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 
 

 

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