产品介绍
| CYT-231 | 重组人制瘤素-M（rHuOSM） | 2μg/10μg/1mg | Prospec |
| Background: | |
| Oncostatin M （OSM） is a member of the interleukin-6 family of cytokines. Of these cytokines, OSM is closely related structually, genetically and functionally to leukemia inhibitory factor. However, OSM-specific biological activities have been reported in hematopoiesis and liver development.
|
|
| Description : | |
| Recombinant Human OSM produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids and having a molecular mass of 26kDa. Recombinant OSM is purified by proprietary chromatographic techniques.
|
|
| Physical Appearance: | |
| Sterile Filtered White lyophilized （freeze-dried） powder.
|
|
| Formulation: | |
| Lyophilized from a concentrated （1mg/ml） solution containg no additives.
|
|
| Solubility: | |
| It is recommended to reconstitute the lyophilized rHuOSM in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
|
|
| Stability: |
|
| Lyophilized Recombinant Human OSM although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution OSM should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
|
|
| Purity: | |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
|
|
| Amino acid sequence: | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Ile-Gly
|
|
| Dimers and aggregates: | |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
|
|
| Biological Activity: | |
| ProSpec’s Human Oncostatin M is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5 x 105 IU/mg.
|
|
| Endotoxin: | |
| Less than 0.1 ng/µg （IEU/µg） of recombinant OSM.
|
|
| Protein content: | |
| Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.45 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
|
|
| Usage: | |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
提供美国光谱医学透析袋，wako生化试剂，amresco生化试剂，sigma生化试剂，优质的分子生物学试剂和试剂盒、培养基本和培养耗材、细胞因子和细胞分离液、抗体和elisa试剂盒及常用生物试剂等,,欢迎登录 或者,，洽谈选购！