Crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.

Description: Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals.

Staining of crystals with appropriate dyes is a very straightforward method to differentiate between macromolecular crystals and salt crystals [1].

Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like JBS True Blue, are able to permeate the solvent channels of a protein, thus coloring the protein blue. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.

Selected References:
[1] Wilkosz et al. (1995) Preliminary characterization of EcoRI-DNA co-crystals: incomplete factorial design of oligonucleotide sequences. Acta Cryst. D 51:938.

Simply add 0.5 μl of JBS Crystal Dye to the crystallization drop containing the crystals of interest.

Coloring Time:
Colors protein crystals after a few minutes. Even if the color of the solution is only faintly blue under the microscope, proteins will be stained within 5-15 min.

Very occasionally, it has been reported that protein crystals did not absorb crystal dyes ^[2].

Safety Information:
Methylene Blue is a harmful substance if swallowed. Work carefully and wear gloves when handling the dye. Under fire conditions it emits toxic fumes.

SKU： M-CO 类别： 结晶优化，结晶筛选和优化
产品信息
应用
用法
晶体染料用于染色大分子晶体，即蛋白质、肽和核酸晶体，以便将它们与小分子和盐晶体区分开来。

描述：结晶筛选与高浓度的沉淀剂和盐可能会导致盐晶体的形成。很难区分这些假阳性和真正的蛋白质晶体。

用合适的染料对晶体进行染色是区分大分子晶体和盐晶体的一种非常简单的方法 [1]。

蛋白质和盐晶体的溶剂含量有很大不同。小晶体染料，如 JBS True Blue，能够渗透蛋白质的溶剂通道，从而使蛋白质呈蓝色。相比之下，盐晶体紧密堆积，没有大的溶剂通道。因此它们将保持无色。

参考文献：
[1] Wilkosz 等。 (1995) EcoRI-DNA 共晶的初步表征：寡核苷酸序列的不完全因子设计。晶体学报。 D 51:938。

只需将 0.5 μl JBS Crystal Dye 添加到含有目标晶体的结晶液滴中。

着色时间：
几分钟后为蛋白质晶体着色。即使溶液的颜色在显微镜下只是淡淡的蓝色，蛋白质也会在 5-15 分钟内被染色。

偶尔，据报道蛋白质晶体不吸收晶体染料 [2]。

安全信息：
如果吞食亚甲蓝是一种有害物质。处理染料时要小心工作并戴上手套。在火灾条件下，它会散发有毒烟雾。

Selected References:
[2] Eckert et al. (2003) Crystallization and preliminary X-ray analysis of Alicyclobacillus acidocaldarius endoglucanase CelA. Acta Cryst. D 59:139.

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 12 months