New England Biolabs supplies a 10X reaction buffer for use with NEBNext^® End Repair Enzyme Mix.

At a 1X concentration this reaction buffer assures optimal activity of the enzyme mix.

1X Buffer Components

50 mM Tris-HCl
10 mM MgCl₂
10 mM DTT
1 mM ATP
0.4 mM dATP
0.4 mM dCTP
0.4 mM dGTP
0.4 mM dTTP
pH 7.5@25°C

-20°C