重组大鼠粒-巨噬细胞集落刺激因子（rrGM-CSF）

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CYT-395 重组大鼠粒-巨噬细胞集落刺激因子（rrGM-CSF）  2μg/10μg/1mg   Prospec

 

 

Background:
  The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterised by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the connections is a two-stranded anti-parallel beta-sheet. The tertiary structure has a topology similar to that of （pig） growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus .

Granulocyte Macrophage Colony Stimulating Factoris produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL-2 beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors.

 
Description :
  Recombinant Rat GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton.
Rat GM-CSF is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.

 
Formulation:
  The protein was lyophilized with no additives.

 
Solubility:
  It is recommended to reconstitute the lyophilized GM-CSF in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Rat GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.01 ng/ml, corresponding to a Specific Activity of 1×108 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of Rat GM-CSF .

 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a calibrated solution of Rat GM-CSF as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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