| CYT-401 | 重组猪粒-巨噬细胞集落刺激因子（rpGM-CSF） | 2μg/10μg/1mg | Prospec |
| Background: | |
| The overall structure of GM-CSF is highly compact and globular with a predominantly hydrophobic core, is characterised by a 4-alpha-helix bundle. The helices are arranged in a left-handed anti-parallel fashion, with two overhand connections. Within the connections is a two-stranded anti-parallel beta-sheet. The tertiary structure has a topology similar to that of （pig） growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus .
Granulocyte Macrophage Colony Stimulating Factor is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. Granulocyte Macrophage-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors , including IL2-beta, IL-3 , IL-6 , IL-7 , EPO and the Prolactin receptors
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| Description : | |
| Recombinant Porcine GM-CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14381 Dalton. Porcine GM-CSF is purified by proprietary chromatographic techniques.
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| Physical Appearance: | |
| Sterile Filtered White lyophilized （freeze-dried） powder.
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| Formulation: | |
| The protein was lyophilized with no additives.
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| Solubility: | |
| It is recommended to reconstitute the lyophilized Porcine GM-CSF in sterile 20mM AcOH （acetic Acid） not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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| Stability: |
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| Lyophilized Porcine GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GM-CSF should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
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| Purity: | |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
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| Amino acid sequence: | |
| The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Arg-Pro.
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| Dimers and aggregates: | |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
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| Biological Activity: | |
| ProSpec’s Porcine GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation TF-1 cell line is < 20 ng/ml.
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| Endotoxin: | |
| Less than 0.1 ng/µg （IEU/µg） of GM-CSF .
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| Protein content: | |
| Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.977 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
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| Usage: | |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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