重组人表皮生长因子（rHuEGF）

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CYT-217 重组人表皮生长因子（rHuEGF）  100μg/500μg   Prospec

 

 

Background:
  A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system.
 
Description :
  Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.
The EGF is purified by proprietary chromatographic techniques.
 
Physical Appearance:
  Sterile Filtered White lyophilized （freeze-dried） powder.
 
Formulation:
  Recombinant EGF was lyophilized from a concentrated （1mg/ml） solution with no additives.
 
Solubility:
  It is recommended to reconstitute the lyophilized EGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
  Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution EGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.
 
Purity:
  Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been compley removed enzymatically.
 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
  ProSpec's recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells （measured by 3H-thymidine uptake） is less then 0.1 ng/ml corresponding to a specific activity of 1 x 107 Units/mg.
 
Endotoxin:
  Less than 0.1 ng/µg （IEU/µg） of recombinant EGF .
 
Protein content:
  Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of human EGF as a Reference Standard.
 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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