The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans,Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae,Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 – 20 µg of DNA with a size distribution of 35 – 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
HIGHLIGHTS
- Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
- Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
- High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
TECHNICAL SPECIFICATIONS
| Applicable For | Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 60 µl TE or DNase-free Water |
| Equipment | Microcentrifuge, heat block/bath |
| Processing Time | 1.5 hours |
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107 cells) |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA. |
| Type | Total DNA |
| Yield | ≤ 25 µg of total DNA |
| Cat # | Name | Size |
|---|---|---|
| D2002 | YeaStar Genomic DNA Kit | 40 Preps |