美国ZYMO RESEARCH-ZymoPURE Plasmid Kits

ZymoPURE Plasmid Kits

ZymoPURE II Plasmid kits are the fastest and simplest plasmid kits available to efficiently isolate transfection-grade plasmid DNA from E. coli (up to 400 µg in 18 minutes). From these plasmid kits, the eluted plasmid DNA is ready for immediate use, without any alcohol precipitation. These plasmid kits feature ZymoPURE II technology, including a modified alkaline lysis system and novel binding chemistry. Together, these plasmid kits yield highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. Instead of time-consuming gravity flow columns, these plasmid kits utilize vacuum filtration and binding for rapid sample processing. As an added convenience, these plasmid kits contain colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The wash regimen of these plasmid kits has been optimized to ensure the eluted DNA is free of endotoxins, salt, protein, and RNA resulting in plasmids that are suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing. Finally, the specially designed spin-columns in these plasmid kits allow for elutions on a microcentrifuge. *Patent Pending

COMPARISON TABLE

CATALOG #

PRODUCT

BINDING CAPACITY

ELUTION VOLUME

CULTURE INPUT

ENDOTOXIN LEVELS

FORMAT

D4208T

ZYMOPURE PLASMID MINIPREP

100 ΜG

≥ 25 ΜL

≤ 5 ML CULTURE

≤ 1 EU/ΜG

SPIN COLUMN

D4202

ZYMOPURE II PLASMID MAXIPREP

1.2 MG

≥ 200 ΜL

≤ 150 ML

≤ 0.025 EU/ΜG DNA

SPIN COLUMN

D4200

ZYMOPURE II PLASMID MIDIPREP

400 ΜG

≥ 100 ΜL

≤ 50 ML

≤ 0.025 EU/ΜG DNA

SPIN COLUMN

D4204

ZYMOPURE II PLASMID GIGAPREP

10 MG

≥ 2 ML

≤ 2.5 L

≤ 0.025 EU/ΜG DNA

SPIN COLUMN

D4213

ZYMOPURE-EXPRESS PLASMID MIDIPREP

1.2 MG

≥ 200 ΜL

25 ML

≤ 1 EU/ΜG PLASMID DNA

SPIN COLUMN

ZymoPURE Plasmid Miniprep Kit

D4208T / D4209 / D4210 / D4211 / D4212

 


 

Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA…

 ZymoPURE II Plasmid Maxiprep Kit

D4202 / D4203

 


 

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade…

ZymoPURE II Plasmid Midiprep Kit

D4200 / D4201


 

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 400 µg of transfection grade…

 ZymoPURE II Plasmid Gigaprep Kit

D4204


 

Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 10 mg of transfection grade…

ZymoPURE – Express Plasmid Midiprep Kit

D4213


 

The ZymoPURE – Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the…