The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coliusing a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
TECHNICAL SPECIFICATIONS
Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
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Elution Volume | ≥ 30 µl |
Endotoxin Levels | < 50 EU/µg |
Equipment | Microcentrifuge |
Processing Time | 15 min |
Purity | Typical Abs260/280 ≥1.8. |
Size Range | Up to 25 kb |
Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Cat # | Name | Size |
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D4054 | ZR Plasmid Miniprep – Classic | 800 Preps |
D4015 | ZR Plasmid Miniprep – Classic | 100 Preps |
D4016 | ZR Plasmid Miniprep – Classic | 400 Preps |