The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coliusing a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
TECHNICAL SPECIFICATIONS
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Endotoxin Levels | < 50 EU/µg |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
| Cat # | Name | Size |
|---|---|---|
| D4054 | ZR Plasmid Miniprep – Classic | 800 Preps |
| D4015 | ZR Plasmid Miniprep – Classic | 100 Preps |
| D4016 | ZR Plasmid Miniprep – Classic | 400 Preps |