The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.

Applicable For	Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications.
Elution Volume	≥ 10 µl
Equipment	Microcentrifuge
Processing Time	15 min
Purity	Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0
Size Range	Up to ~200 kb
Yield	Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coliculture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture.