DESCRIPTION
The ZR BAC DNA Miniprep Kit efficiently isolates BAC plasmid DNA or other large plasmids (e.g., PAC) from E. Coli using a procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol with color-coded reagents that allow easy visualization of complete bacterial cell lysis and neutralization. The innovative Zymo-Spin™ IC-XL columns are optimized for high yield, endotoxin-free plasmid DNA recovery. BAC DNA purified using the ZR BAC DNA Miniprep Kit is ideal for sequencing, PCR, endonuclease digestion, etc.
TECHNICAL SPECIFICATIONS
Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
---|---|
Elution Volume | ≥ 10 µl |
Equipment | Microcentrifuge |
Processing Time | 15 min |
Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
Size Range | Up to ~200 kb |
Yield | Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coliculture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture. |
Cat # | Name | Size |
---|---|---|
D4048 | ZR BAC DNA Miniprep Kit | 25 Preps |
D4049 | ZR BAC DNA Miniprep Kit | 100 Preps |