The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the “crush and soak” method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.

 

 

• Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
• Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
• Compatible with up to 25% (w/v) polyacrylamide.

Equipment	Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer
Purity	RNA is ready for all subsequent analysis and molecular manipulation
Sample Source	RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).
Size Range	RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)
Yield	10 µg RNA (binding capacity), ≥6 µl (elution volume)

Cat #	Name	Size
R1070	ZR small-RNA PAGE Recovery Kit	20 Preps