从250-500mL菌液中提取质粒DNA |
说明书点击下载 |
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产品特性– 45分钟快速提取高质量质粒DNA – 获得的高质量DNA可直接进行酶切、测序、标记或PCR – 采用了大容量硅胶离心柱 – 从标准培养或高营养培养基都能获得大量DNA 描述 UltraClean® Maxi Plasmid Prep Kit专门设计用于提取大体积 先由改进配方的碱性裂解液裂解细菌,DNA吸附到硅胶离心柱滤膜上,经洗涤重悬与小体积的缓冲液中。更终DNA浓度较大,可直接进行自动测序,或进行酶切、标记、PCR等下游使用。 研究与深入
条带2-5:UltraClean® Maxi Plasmid Prep Kit 提取了的几个菌液的pUC19 |
规格
保存条件室温保存2年。 文献
客户评价Best Value for the Money; Best Yield for the Time. After using other maxiprep kits, including those from Clontech, Qiagen, Sigma and BioRad (both the Quantum Prep and the new Aurum) kits, as well as doing the protocol straight from Sambrook, I can only say that your kit gives me the best, purest, highest yield per dollar in the least amount of time. Sure, the package is not as pretty as the other kits, but as a very busy research associate/lab mommy I just do not have time to be making maxipreps time after time or to spend two or three days completing them. I do not have time to spend on additional purifications to get a “glassy” pellet that actually goes into solution properly. Your kit is the best kit for my needs. Lisa Miller
Great, Clean DNA. This was my first time using a MO BIO product. I needed to generate a relatively large amount of a low copy plasmid. I used the MO BIO TB DRY® Powder Growth Media to grow 250 ml of E.coli containing my plasmid. Then I used the maxi plasmid kit and generated approximately 80 µg of my low copy plasmid. This was an excellent yield for this construct. The DNA was sent out for sequencing and so far our results from that are beautiful also. I strongly recommend this kit for all ultra-clean plasmid preps. hscott@anl.gov 试剂盒组分
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