产品特性
– 提示： UltraClean® Standard Mini Plasmid Prep Kit升级了！DNA得率、纯度更高，提取速度更快！
– 10分钟快速提取高质量DNA，可直接用于酶切、测序、标记或PCR
– 采用常规的试剂和熟悉的硅胶离心柱
– 从1-5ml菌液就能提到达20μg的DNA
– 适合TB DRY® Powder Growth Media培养的低拷贝数菌液
描述
UltraClean® Standard Mini Plasmid Prep Kit使用了大多数质粒提取用到的碱性裂解液裂解技术。
操作中使用的试剂量跟其它品牌试剂盒一样，无需重新学习。改良了剂配方和缩短了离心时间，整个提取操作省时、省事！采用的硅胶离心柱可更大程度地回收到测序级的高质量质粒。
适用于LB和所有高营养培养基，提取的质粒不含基因组DNA。且浓度足以直接用来进行自动测序，也可以直接进行酶切、连接、PCR和其它下游应用。
|
规格
| 方法 |
硅胶离心柱 |
| 细胞裂解 |
碱性裂解液裂解 |
| 滤膜DNA吸附能力 |
20 µg每孔 |
| 通量 |
1 – 24个样 |
| 操作时间 |
20 分钟 |
| 仪器设备 |
微型离心机 |
文献
- Wells, C.A., Salvage-Jones, J.A., Li, X., Hitchens, K., Butcher, S., Murray, R.Z., Beckhouse, A.G., Lo, Y., Manzanero, S., Cobbold, C., Schroder, K., Ma, B., Orr, S., Stewart, L., Lebus, D., Sobieszczuk, P., Hume, D.A., Stow, J., Blanchara, H., and Ashman, R.B. The Macrophage-Inducible C-Type Lectin, Micle is and Essential Component of the Innate Immune Response to Candida albicans. The Journal of Immunology. 180: 7404-7413 (2008).
- Morin, P.A., Hedrick, N.M., Robertson, K.M., Leduc, C.A. Comparative Mitochondrial and Nuclear Quantitative PCR of Historical Marine Tissue, Bone, Baleen, and Tooth Samples . Molecular Ecology Notes. (7) 3: 404-411 (2007).
- Ulrich, P.N., Colton, C.M., Hoover, C.A., Gaffney, P.M., Marsh, A.G. Haplosporidium Nelsoni rDNA Detection in Oysters from the Gulf of Mexico and the Caribbean Sea. Journal of Shellfisheries Research. (2007).
试剂盒组分
| 组分 |
12301-50 |
12301-100 |
12301-250 |
Solution 1
Solution 2
Solution 3
Solution 4
Solution 5
Collection Tubes
Spin Filters In Tubes |
1 x 14 ml
1 x 14 ml
1 x 20 ml
2 x 30 ml
1 x 3 ml
50
50 |
1 x 28 ml
1 x 28 ml
1 x 39 ml
3 x 30 ml
1 x 6 ml
100
100 |
1 x 69 ml
1 x 69 ml
1 x 97 ml
1 x 97 ml
1 x 15 ml
250
250 |
|
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