美国MOBIO 土壤DNA提取试剂盒(UltraClean® Soil DNA Isolation Kit)

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UltraClean® Soil DNA Isolation Kit

Overview

For isolating genomic DNA from routine soil and fecal samples

Order
Description Catalog # Price
50 preps 12800-50 N/A
100 preps 12800-100 N/A

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UltraClean® Fecal DNA Isolation Kit

Features

– Established method selectively removes most PCR inhibitors

– Rapidly purify DNA from up to one gram of soil in 30 minutes

– Yields high quality PCR ready DNA up to 50 kb plus

– Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR

– Efficiently lyses spores and fungi; also compatible with fecal samples

Description

The UltraClean® Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.

Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.

This is our original DNA kit for soil – for more challenging soil samples we recommend the PowerSoil® DNA Isolation Kit

 

 NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

Research and Development


Purification of DNA from various soils using
the UltraClean® Soil DNA Isolation Kit
 
美国MOBIO 土壤DNA提取试剂盒(UltraClean® Soil DNA Isolation Kit)

  M 1 2 3 4  

        Lane 1: Forest soil
        Lane 2: Bog soil
        Lane 3: Pristine sand
        Lane 4: Farm soil

 

PCR amplification of DNA extracted with this soil kit using universal eubacterial primers
 
美国MOBIO 土壤DNA提取试剂盒(UltraClean® Soil DNA Isolation Kit)

1 2 3 4 5 6 7 8

        Lane 1: Marker
        Lane 2: Negative control
        Lanes 3-8: Six different soil types

 

Learn more about the applications of the UltraClean® Soil DNA Isolation Kit in this poster presented at the American Society for Microbiology Meeting.

Testing the UltraClean® Soil DNA Isolation Kit
on a Diverse Range of Soils
by PCR Amplification of 16S rDNA.

Download Here (pdf 872KB)

 

美国MOBIO 土壤DNA提取试剂盒(UltraClean® Soil DNA Isolation Kit)
Click over image to enlarge

MSDS

12800

Protocol

12800
 

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per filter
Throughput 1 – 24 samples
Time 30 minutes
Equipment Required Vortex Adapter
Positive PCR Results Obtained
with these Soil Types
  • Clay
  • Fecal/Intestinal Tract
  • Fungal Mat
  • Forest
  • Loam
  • Sand
  • Potting Soil
  • Petroleum Saturated

Publications

  • Andreote, F., Carneiro, R., Salles, J., Marcon, J., Labate, C., Azevedo, J., and Araujo, W. Culture-Independent Assessment of Rhizobiales-Related Alphaproteobacteria and the Diversity of Methylobacterium in the Rhizosphere and Rhizoplane of Transgenic Eucalyptus. Microbial Ecology. 57: 82-93 (2009).
  • Baniulyte, D., Favila, E., and Kelly, J.Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediment. Microbial Ecology. 57: 160-169 (2009).
  • Barkovskii, A., Fukui, H., Leisen, J., Kim, S., Marsh, T., and Khijniak, A. Rearrangement of Bacterial Community Structure During Peat Diagenesis. Soil Biology and Biochemistry. 41: 135-143 (2009).

Customer Comments

I consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean® Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.
 

      Elise C. Poole
      Michigan State University
      Dept. of Botany and Plant Pathology

      Read More Customer Comments
      on the UltraClean® Soil DNA Kit

Kit Components

Component 12800-50 12800-100
Bead Tubes
Solution S1
IRS Solution
Solution S2
Solution S3
Solution S4
Solution S5
Spin Filters In Tubes
Collection Tubes		 50
1 x 3.3 ml
1 x 11 ml
1 x 14 ml
1 x 72 ml
1 x 16.5 ml
1 x 3 ml
50
150		 100
1 x 6.6 ml
1 x 22 ml
1 x 27.5 ml
1 x 143 ml
1 x 30 ml
1 x 6 ml
100
300