Crystal Screens – JBScreen BasicDespite intensive research, the crystallization of biological macromolecules remains a process of trial and error. Nucleation and crystal growth are influenced by the interaction of many variables, such as temperature, pH, precipitant and salt concentration.
Testing all possible combinations would be too time consuming and would require enormous amounts of sample. One approach to find suitable crystallization conditions is the sparse-matrix method. This method involves screening with an intentional bias towards conditions which have been proven successful in the crystallization of biological macromolecules.In 1991, Jancarik and Kim published 50 conditions, which were derived from previously crystallized proteins [1]. These and other conditions form the basis of the JBScreen Basic system [1,2]. However, JBScreen Basic is designed to fit the 24-well plate format and like in all other JBScreen crystallization kits, we abstained from the use of cacodylate buffers and replaced them with MES. JBScreen Basic contains 96 unique reagent mixtures for screening a wide range of pH and various salts and precipitants.

Format

Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well

References

[1] Jancarik and Kim (1991) Sparse matrix sampling: a screening method for crystallization of proteins. J. Appl. Cryst. 24:409.
[2] Cudney et al. (1994) Screening and optimization strategies for macromolecular crystal growth. Acta Cryst. D 50:414.

Selected Recent Literature Citations of JBScreen Basic

• Moonens et al. (2015) Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies. Veterinary Research 46:14.
• Zano et al. (2014) Structure of an unusual S-adenosylmethionine synthetase from Campylobacter jejuni. Acta Cryst. D 70:442.
• Goyal et al. (2013) Crystallization and preliminary X-ray crystallographic analysis of the curli transporter CsgG. Acta Cryst. F 69:1349.

Download technical sheet from below:JBScreen Basic 1 JBScreen Basic 2 JBScreen Basic 3 JBScreen Basic 4 JBScreen Basic HTS L

类别：结晶筛
产品信息
产品描述
规格 – 屏幕条件
水晶屏 – JBScreen Basic
尽管进行了深入的研究，但生物大分子的结晶仍然是一个反复试验的过程。成核和晶体生长受许多变量相互作用的影响，例如温度、pH、沉淀剂和盐浓度。
测试所有可能的组合会非常耗时，并且需要大量的样本。寻找合适结晶条件的一种方法是稀疏矩阵法。该方法涉及有意偏向已证明在生物大分子结晶中成功的条件的筛选。

1991 年，Jancarik 和 Kim 发表了 50 个条件，这些条件源自先前结晶的蛋白质 [1]。这些和其他条件构成了 JBScreen Basic 系统 [1,2] 的基础。然而，JBScreen Basic 设计为适合 24 孔板格式，与所有其他 JBScreen 结晶试剂盒一样，我们放弃使用二甲脒酸盐缓冲液并用 MES 代替。 JBScreen Basic 包含 96 种独特的试剂混合物，用于筛选各种 pH 值和各种盐和沉淀剂。

格式

散装 – 24 或 96 种筛查溶液，10 毫升等分试样
HTS – 96 种筛选溶液以深孔模块形式提供，每孔 1.7 ml

参考

[1] Jancarik 和 Kim (1991) 稀疏矩阵采样：蛋白质结晶的筛选方法。 J. 应用程序水晶。 24:409。
[2] 卡德尼等人。 (1994) 大分子晶体生长的筛选和优化策略。晶体学报。 D 50:414。

JBScreen Basic 近期文献引用选编

穆恩斯等人。 (2015) 美洲驼单域抗体抑制 F4 菌毛粘附产生肠毒的大肠杆菌的结构洞察。兽医研究 46:14。
扎诺等人。 (2014) 来自空肠弯曲杆菌的不寻常 S-腺苷甲硫氨酸合成酶的结构。晶体学报。 D 70:442。
戈亚尔等人。 (2013) 卷曲转运蛋白 CsgG 的结晶和初步 X 射线晶体学分析。晶体学报。 F 69:1349。
从下面下载技术表：
JBScreen Basic 1 JBScreen Basic 2 JBScreen Basic 3 JBScreen Basic 4 JBScreen Basic HTS L