JBScreen PACT++ is a crystallization screen facilitating systematic pH, anion- and cation testing in the presence of polyethylene glycol (PEG) based on the work of Newman et al. [1].The 96 unique crystallization conditions combine three mini-screens in one:

1. 24-condition PEG/pH screen
2. 24-condition PEG/cation screen
3. 48-condition PEG/anion screen

This systematic approach aims to alter individual components of the crystallization conditions, i.e. pH, anions and cations, independently from the others in order to obtain more information of the precipitation behaviour of the protein.

When JBScreen PACT++ is used along with JBScreen JCSG++, systematic investigation of the precipitation behaviour of the protein can be combined with a sparse matrix screen in order to enhance the success rate of protein crystallization.

Format

Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well

类别：结晶筛
产品信息
产品描述
规格 – 屏幕条件
JBScreen PACT++ 是基于 Newman 等人的工作在聚乙二醇 (PEG) 存在下促进系统 pH、阴离子和阳离子测试的结晶屏幕。 [1]。
96 种独特的结晶条件将三个迷你屏幕合二为一：

1. 24 条件 PEG/pH 筛选
2. 24 条件 PEG/阳离子筛选
3. 48 条件 PEG/阴离子筛网

这种系统方法旨在独立于其他因素改变结晶条件的各个组成部分，即 pH 值、阴离子和阳离子，以获得有关蛋白质沉淀行为的更多信息。

当 JBScreen PACT++ 与 JBScreen JCSG++ 一起使用时，蛋白质沉淀行为的系统研究可以与稀疏矩阵筛选相结合，以提高蛋白质结晶的成功率。

格式

散装 – 24 或 96 种筛查溶液，10 毫升等分试样
HTS – 96 种筛选溶液以深孔模块形式提供，每孔 1.7 ml

References

[1] Newman et al. (2005) Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D 61:1426.

Download technical sheet from below:JBScreen PACT ++ 1 JBScreen PACT ++ 2 JBScreen PACT ++ 3 JBScreen PACT ++ 4 JBScreen PACT++ HTS L