Crystal Growth and more..

The CrystalDirect™ plate is an ideal support for crystal growth, in situ XRD, serial data collection and in situ soaking experiments. It is particularly well suited for work with microcrystals, where low background scattering is essential.

in situ X-ray diffraction

The CrystalDirect™ plate is a 96-well vapor diffusion crystallization plate that uses an ultrathin film as crystallization support (25 micron and lower). It provides extremely low x-ray background for diffraction measurements and high transparency to both visible and UV light for drop inspection.

*An ultrathin top sealing film is also provided to further reduce background scattering.

 Automated Crystal Harvesting

The CrystalDirect™ plate is compatible with standard crystallization robots and imagers. Furthermore, in combination with the ARINAX CrystalDirect® harvester robot the CrystalDirect™ plates enable fully automated crystal harvesting and cryocooling.

CrystalDirect® is able to harvest protein crystals from 96-well CrystalDirect^TM plates by means of laser photoablation. The protein crystals are directly mounted on a SPINE compatible sample pin. Then, they are placed into a cryo-stream and made available to be transferred into a cryostorage (manually or via sample changer robot).

Learn more about all of the incredible capabilities of the CrystalDirect® system here: www.arinax.com

The robotic equipment for the CrystalDirect® approach has been further developed by Arinax. See videos and learn more here: Arinax.com CrystalDirect®

For any questions about other properties of CrystalDirect Plates please call +1 607 266 877 or email [email protected]

G.A Bezerra et al., Nature (2017), Scientific Reports 7: 2848 , « Bacterial protease uses distinct thermodynamic signatures for substrate recognition

U. Zander, G. Hoffmann, I. Cornaciu, J.-P. Marquette, G. Papp, C. Landret, G. Seroul, J. Sinoir, M. Röwer, F. Felisaz, S. Rodriguez-Puente, V. Mariaule, P. Murphy, M. Mathieu, F. Cipriani and J. A. Márquez (2016). Automated harvesting and processing of protein crystals through laser photoablation Acta Cryst. D72, 454-466

Marquez, J. A., and Cipriani, F. (2014) CrystalDirect: a novel approach for automated crystal harvesting based on photoablation of thin films. Methods in molecular biology 1091, 197-203

Svensson, O., Malbet-Monaco, S., Popov, A., Nurizzo, D. & Bowler, M. W. (2015) Fully automatic characterization and data collection from crystals of biological macromolecules Acta Cryst. D71, 1757-1767.

Cipriani, F., Rower, M., Landret, C., Zander, U., Felisaz, F., and Marquez, J. A. (2012) CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films. Acta Crystallogr D Biol Crystallogr 68, 1393-1399

MiTeGen has established a partnership with the European Molecular Biology Laboratory (www.embl.org) to propose a new generation of crystallization plates that bridges crystallization and x-ray data collection via the CrystalDirect™ approach [1,2,3]. Designed for automated crystal harvesting the CrystalDirect™ plates are also ideal for In-Situ data collection and manual harvesting.